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ytpB [2019-01-30 13:27:27]
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ytpB [2019-01-30 13:27:27]

tetraprenyl-beta-curcumene synthase, catalyzes the first committed step in C35 terpenoid biosynthesis, salt stress protein
locus
BSU30500
pI
5.76
mw
42.68 kDa
protein length
367 aa Sequence Blast
gene length
1104 bp Sequence Blast
function
C35 terpenoid biosynthesis
product
tetraprenyl-beta-curcumene synthase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

    • Gene

    Coordinates
    3,121,538 3,122,641

    Phenotypes of a mutant

  • increased sensitivity to bacitracin, due to the accumulation of heptaprenyl pyrophosphate [Pubmed|24806199]
  • sensitivity is increased in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB] [gene|34DBBDD76A0B36867E5116FF17391DF70062F745|menA]'' double mutant [Pubmed|24806199]

    • The protein

    Structure

  • [PDB|5YO8] (50% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
    description
    [Pubmed|9387221]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
    • view in new tab

    genes
    [gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]-[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
    description
    [Pubmed|18179421]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A164 (ytpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/164 NBRP B. subtilis, Japan]
  • BKE30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC
  • BKK30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC

    • References

  • 9387221,18179421,23748782,21627333,24806199